Metabarcoding is the identification of the community composition via analysis of the sequences of barcode marker genes and metagenomics is the analysis of the collective genomes of the community. These two methods provide direct access to the enormous genetic diversity of the of soil and have become an essential part of many soil biology studies. Most of these studis have been made for identifying microorganisms presence in the soil, only a few studies have been made with eukaryotic species and any of them where made with a temporal serie. We pretend to describe a new field for this new techniques, the metapaleogenomics applied to invasive species.
In our study we will be able to analyze a 313bp section of the standard DNA barcoding region of the cytochrome c oxidase subunit I (COI) gene using primers described in Leray, et al.; and for metagenomics a variable length target of the V4 nuclear small subunit ribosomal DNA of 18S gene using primers from Amaral-Zettler et al. Tese two primer sets allow for broad characterisation of marine metazoan diversity.